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KMID : 1039120180070010082
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Clinical and Experimental Vaccine Research
2018 Volume.7 No. 1 p.82 ~ p.86
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Evaluation of two different enzyme-linked immunosorbent assay for severe fever with thrombocytopenia syndrome virus diagnosis
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Yu Min-Ah
Jeong Hye-Won
Park Su-Jin
Kim Young-Il
Kim Eun-Ha
Si Young-Jae
Yu Kwang-Min
Robles Norbert John C.
Han Hae-Jung
Choi Young-Ki
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Abstract
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To develop the large scale serological assay for severe fever with thrombocytopenia syndrome virus (SFTSV) infection, we evaluated two different enzyme-linked immunosorbent assay (ELISA) methods using nucleocapsid protein (NP) and Gn proteins of CB1 (genotype B) SFTSV strains. The NP-based ELISA tests showed more sensitive with broad cross-reactivity between two different genotype A and B strains compared with those of Gn-based ELISA tests. However, Gn-based ELISA showed more genotype specificity and specificity. These result suggested that NP-based ELISA test could be applicable for general sero-prevalence studies of SFTSV infections, while Gn-based ELISA could be applicable for a certain specific genotype sero-prevalence study.
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KEYWORD
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Severe fever with thrombocytopenia syndrome virus, Enzyme-linked immunosorbent assay, NP protein, Gn protein
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